PP19 Development of a method for chlorophyll assessment by a reference from Hibiscus rosa-sinensis
DOI:
https://doi.org/10.3407/qwjsnh19Keywords:
Chlorophyll, Pheophytin a, Hibiscus rosa-sinensis, Lactuca Sativa L.var. longifola, Phytotoxicity, AllelopathyAbstract
In this study, a chlorophyll estimation method was developed using pheophytin a, obtained from Hibiscus rosa-sinensis, as a reference. Pigments were extracted with acetone, and a calibration curve was generated by UV-Vis spectrometry and fluorometry. The method was applied in phytotoxicity and allelopathy tests with Lactuca sativa L. var. longifolia, correlating chlorophyll's presence with the plants' physiological activity. The viability of H. rosa-sinensis as an economical and accessible reference was demonstrated.
References
[1] PETROVIC, S., et al. (2012). The Identification of Chlorophyll and Its Derivatives in the Pigment Mixtures: Hplc-Chromatography, Visible and Mass Spectroscopy Studies. Savremene tehnologije 1: 16-24. URL
[2] LEFEBVRE, T., et al. (2020). Evaluation of the Extraction and Stability of Chlorophyll-Rich Extracts by Supercritical Fluid Chromatography. Anal Bioanal Chem 412(26): 7263-7273. [DOI]
[3] TANIGUCHI, M. y LINDSEY, J. S. (2021). Absorption and Fluorescence Spectral Database of Chlorophylls and Analogues. Photochem Photobiol 97(1): 136-165. [DOI]
[4] DAS, A., et al. Chlorophyll and Chlorophyll Catabolite Analysis by Hplc. In: GUO, Y. (ed.). Plant Senescence: Methods and Protocols. New York, NY: Springer New York, (2018). p. 223-235. [DOI]
[5] VAN BREEMEN, R. B., et al. (1991). Identification of Chlorophyll Derivatives by Mass Spectrometry. Journal of Agricultural and Food Chemistry 39(8): 1452-1456. [DOI]
[6] PETROVIC, S., et al. (2014). Chlorophyll a and Chlorophyllide a inside Liposomes Made of Saturated and Unsaturated Lipids: A Possible Impact of the Lipids Microenvironment. Acta Periodica Technologica 10.2298/apt1445215p(45): 215-227. [DOI]
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