PP45 Evaluation of Antioxidant Capacity and Optimization of Chromatographic Fingerprints by HPLC-DAD-FLD of Extracts from Medicinal Plants.
DOI:
https://doi.org/10.3407/rpn.v6i1pp45Keywords:
antioxidant activity, plant extracts, flavonoids, hplc, PolyphenolsAbstract
Oxidative stress occurs due to an imbalance between the production of reactive oxygen species (ROS) and the antioxidant system's ability to neutralize them. This leads to protein carbonylation and contributes to various diseases. This study evaluated the antioxidant capacity of extracts from six endemic plants of San Juan de Nepomuceno. Using proteomic, chromatographic, and spectroscopic methods, the plants Limonaria, Rosemary, Chuchuguaza, Annatto, Sage, and Coca were identified with high content of polyphenols and flavonoids. The results showed significant antioxidant activity by inhibiting protein carbonylation across all extracts, and useful chromatographic patterns were obtained for future studies.
References
[1] De Almeida AJPO, De Oliveira JCPL, Da Silva Pontes LV, De Souza Júnior JF, Gonçalves TAF, Dantas SH, et al. ROS: Basic Concepts, Sources, Cellular Signaling, and its Implications in Aging Pathways. Vol. 2022, Oxidative Medicine and Cellular Longevity. Hindawi Limited; 2022.
[2] Shen, N., Wang, T., Gan, Q., Liu, S., Wang, L., & Jin, B. (2022). Plant flavonoids: Classification, distribution, biosynthesis, and antioxidant activity. Food chemistry, 383, 132531.
[3]Marrugo-Padilla, Darío Méndez-Cuadro, Erika Rodríguez-Cavallo, Tetracycline residues induce carbonylation of milk proteins and alter their solubility and digestibility, International Dairy Journal, Volume 125, 2022, 105226, ISSN 0958- 6946. https://doi.org/10.1016/j.idairyj.2021.105226.
[4] Akbar A, Gul Z, Chein SH, Sadiq MB. Investigation of Anti-Inflammatory Properties, Phytochemical Constituents, Antioxidant, and Antimicrobial Potentials of the Whole Plant Ethanolic Extract of Achillea santolinoides subsp. wilhelmsii (K. Koch) Greuter of Balochistan. Oxid Med Cell Longev. 2023;2023.
[5] De la Rosa Cogollo, I., Marrugo-Padilla, A., Rodríguez-Cavallo, E., & Méndez-Cuadro, D. (2020). In natura tropical juices inhibit the in vitro carbonylation of bovine serum albumin. Fruits, 75(6), 247-257.
[6] De Magalhães BEA, Dos Santos WNL. Phenolic content and antioxidant capacity of infusions herbs: Optimization of phenolic extraction and HPLC-dad method. An Acad Bras Cienc. 2020;92(3):1–17.
[7] Li, Y., He, Z., Shao, N., Xu, Z., Rong, S., & Zhu, X. (2024). Establishment of multivariate digital high performance liquid chromatography fingerprints of fruit juice essence and its flavor quality evaluation. Microchemical Journal, 200, 110402
Downloads
Published
Issue
Section
Categories
License
Copyright (c) 2025 Yissed Giraldo-Naranjo, Karol Pérez-Reales, Dilia Mejía , Aracelys Rodríguez , Gabriel Medina, Dr. Darío Méndez-Cuadro, Erika Rodríguez-Cavallo
This work is licensed under a Creative Commons Attribution 4.0 International License.
License Terms
The Creative Commons Attribution License (CC BY 4.0) allows users to copy, distribute and transmit an article and adapt the article and make commercial use of the article. The CC BY license permits commercial and non-commercial re-use of an open access article, as long as the author is properly attributed.
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
- You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.
- No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity.
How to Cite
