PO32 Phytochemical study of Siparuna gesnerioides and its antiviral activity against SARS-CoV-2

Authors

  • Jhon Fredy Castañeda Universidad Surcolombiana https://orcid.org/0000-0003-0434-2676
  • Gilda G. Leitão Instituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro.
  • Suzana G. Leitão Programa de Biotecnologia Vegetal e Bioprocessos, Universidade Federal do Rio de Janeiro, Rio de Janeiro
  • Diego Allonso Programa de Biotecnologia Vegetal e Bioprocessos, Universidade Federal do Rio de Janeiro, Rio de Janeiro
  • Thamires Fonseca Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio de Janeiro, Rio de Janeiro
  • Mariana Campos Programa de Biotecnologia Vegetal e Bioprocessos, Universidade Federal do Rio de Janeiro, Rio de Janeiro
  • Simony Mendoca Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro
  • Esthella Silva Instituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro
  • Nayara Sales Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio de Janeiro, Rio de Janeiro
  • Diegina Fernándes Instituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro.

DOI:

https://doi.org/10.3407/hmp1mc74

Keywords:

Siparunaceae, Siparuna gesnerioides, Countercurrent Chromatography, Nuclear Magnetic Resonance, Antiviral Potential for Covid-19.

Abstract

Species of the genus Siparuna (Siparunaceae) are popularly recognized for their anxiolytic, antioxidant, and antiviral properties1,2. 

The most active extract was purified by countercurrent chromatography (CCC) using an HTPrep system equipped with a 112 mL column, using a hexane-ethyl acetate-methanol-water solvent system in the ratios 3:7:5:5 and 5:5:5:5 v/v. TFraction XIII was selected for purification, allowing the identification of the flavonoid 3,7-di-O-methyl-kaempferol (kumatakenin). The extract showed a 54% inhibition of the SARS-CoV-2 RBD:ACE2 interaction and a 92% inhibition in the PL pro and 3CLpro assays.

References

[1] LEITÃO, G. G., et al. (2000). Kaempferol Glycosides from Siparuna Apiosyce. Phytochemistry 55(6): 679-682. [DOI]

[2] LEAL, C. M., et al. (2022). Bioassay-Guided Fractionation of Siparuna Glycycarpa n-Butanol Extract with Inhibitory Activity against Influenza A(H1N1)Pdm09 Virus by Centrifugal Partition Chromatography (CPC). Molecules 27(2). [DOI]

[3] GONZALEZ, J. E. A., et al. (2022). Aislamiento, Caracterización y Evaluación de los Aceites Esenciales de Siparuna gesnerioides (Kunth) A. DC., como Agente Antimicrobiano contra Salmonella spp. Revista Productos Naturales, 5(2), 179-181. [DOI]

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Published

2025-06-25

Issue

Vol. 6 No. 1 (2025): I Colombian Congress of Natural Products (2025)

Section

Abstract

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License

Copyright (c) 2025 Jhon F. Castañeda-Goméz, Gilda G. Leitão, Dra. Suzana G. Leitão, Dr. Diego Allonso, Dra. Thamires Fonseca, Dra. Mariana Campos, Dra. Simoni Mendoca, Dra. Esthella Silva, Nayara Sales, Dra. Diegina Fernándes

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How to Cite

PO32 Phytochemical study of Siparuna gesnerioides and its antiviral activity against SARS-CoV-2. (2025). Revista Productos Naturales, 6(1), 123-125. https://doi.org/10.3407/hmp1mc74