PP57 Heterogeneous Photocatalysis with TiO2 for the Oxidation of Synthetic Lipids from Staphylococcus aureus: Optimization and Biophysical Implications

Authors

  • Juan Esteban Gonzalez Martinez Universidad de Los Andes image/svg+xml , University of Los Andes image/svg+xml , University of the Andes image/svg+xml
  • Chad Leidy Laboratory of Biophysics, Physics Department, Universidad de los Andes, Bogotá 111711, Colombia
  • Chiara Carazzone Laboratory of Advanced Analytical Techniques in Natural Products (LATNAP), Chemistry Department, Universidad de los Andes, Bogotá 111711, Colombia

DOI:

https://doi.org/10.3407/rpn.v6i1pp57

Keywords:

Heterogeneous Photocalysis, S. aureus, Oxidation, Biophysical Properties, Experiment of Design, Membrane

Abstract

Oxidative stress affects the structure and function of bacterial membranes, influencing their stability and resistance. In this study, heterogeneous photocatalysis with TiO₂ was used to induce oxidation in synthetic lipids of Staphylococcus aureus. Through a design of experiments, hydroxyl radical generation was optimized, identifying TiO₂ concentration and agitation as key factors. The results showed changes in membrane rigidity and phase transition temperature, indicating structural alterations. These findings could contribute to the development of antimicrobial strategies and applications in biomedicine and environmental decontamination.

References

[1] SIES, H., et al. (2017). Oxidative Stress. Annual Review of Biochemistry 86(Volume 86, 2017): 715-748. [doi]

[2] YONG, S.-S., et al. (2023). Tio2-Based Photocatalyst Generated Reactive Oxygen Species Cause Cell Membrane Disruption of Staphylococcus Aureus and Escherichia Coli O157:H7. Food Microbiology 109: 104119. [doi]

[3] MANESS, P.-C., et al. (1999). Bactericidal Activity of Photocatalytic Tio2 Reaction: Toward an Understanding of Its Killing Mechanism. Applied and Environmental Microbiology 65(9): 4094-4098. [doi]

[4] MARINAKI, M., et al. (2023). Development of Two-Level Design of Experiments for the Optimization of a Hs-Spme-Gc-Ms Method to Study Greek Monovarietal Pdo and Pgi Wines. Talanta 253: 123987. [doi]

[5] DEMARS, Z., et al. (2020). Exogenous Fatty Acids Remodel Staphylococcus Aureus Lipid Composition through Fatty Acid Kinase. Journal of Bacteriology 202(14): 10.1128/jb.00128-00120. [doi]

[6] KENNY, J. G., et al. (2009). The Staphylococcus Aureus Response to Unsaturated Long Chain Free Fatty Acids: Survival Mechanisms and Virulence Implications. PLOS ONE 4(2): e4344. [doi]

[7] SEN, S., et al. (2016). Growth-Environment Dependent Modulation of Staphylococcus Aureus Branched-Chain to Straight-Chain Fatty Acid Ratio and Incorporation of Unsaturated Fatty Acids. PLOS ONE 11(10): e0165300. [doi]

[8] RASKOVIC, D., et al. (2025). Growth of Staphylococcus Aureus in the Presence of Oleic Acid Shifts the Glycolipid Fatty Acid Profile and Increases Resistance to Antimicrobial Peptides. Biochimica et Biophysica Acta (BBA) - Biomembranes 1867(1): 184395. [doi]

Downloads

Published

2026-01-21

How to Cite

Gonzalez Martinez, J. E., Leidy, C., & Carazzone, C. (2026). PP57 Heterogeneous Photocatalysis with TiO2 for the Oxidation of Synthetic Lipids from Staphylococcus aureus: Optimization and Biophysical Implications. Revista Productos Naturales, 6(1), 384-386. https://doi.org/10.3407/rpn.v6i1pp57